Application

Research CategorySignaling

Western blot: 1:1000 ELISA: 1:2000

Anti-N-WASP Antibody is an antibody against N-WASP for use in ELISA & WB.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.

Immunogen

Synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminus of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP sequence.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity-purified antibody is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Specificity

This antibody detects a 65 kDa protein corresponding to the molecular weight of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa WASP protein in Jurkat cell lysate.

Storage and Stability

Maintain at -20°C for up to one year from date of receipt. Do not aliquot.